Improved Assay for Rhodanese in Thiobacillus spp
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چکیده
منابع مشابه
Improved Assay for Rhodanese in Thiobacillus spp.
Rhodanese (thiosulfate:cyanide sulfurtransferase; EC 2.8.1.1) catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. Conventional rhodanese assays colorimetrically measure the formation of one or the other of the products. These assays suffer from the fact that there is significant nonbiological formation of these products in addition to the enzymatically catalyzed reac...
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1. Rhodanese has been extracted from Thiobacillus denitrificans by ultrasonic disintegration of the cells. 2. Studies with Sephadex columns have shown that the enzyme aggregates, forming a tetramer. 3. The molecular weights of the monomer and of an enzymically active sub-unit one-quarter this size have been determined by gel filtration. 4. Higher-molecular-weight forms of rhodanese are broken d...
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Chemical studies of rhodanese (thiosulfate : cyanide sulfurtransferase, EC 2.8.1.1) in this laboratory have prompted the development of a modified procedure for preparing the crystalline enzyme. The new method, which is based on the earlier procedure of S6rbo (1) as modified by Westley et al. (2-4), permits isolation in apparently monodisperse form of nearly 50% of the total rhodanese activity ...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1988
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.54.11.2866-2867.1988